- 抑制劑
- 研究領(lǐng)域
- PI3K/Akt/mTOR信號(hào)通路
- 表觀遺傳
- 甲基化
- 免疫&炎癥
- 酪氨酸蛋白激酶
- 血管生成
- 凋亡
- 自噬
- 內(nèi)質(zhì)網(wǎng)應(yīng)激&UPR響應(yīng)
- JAK/STAT信號(hào)通路
- MAPK信號(hào)通路
- 細(xì)胞骨架
- 細(xì)胞周期
- TGF-beta/Smad信號(hào)通路
- DNA損傷/DNA修復(fù)
- 化合物庫(kù)
- 抗體
- 生物試劑
- qPCR
- 2x SYBR Green qPCR Master Mix
- 2x SYBR Green qPCR Master Mix(Low ROX)
- 2x SYBR Green qPCR Master Mix(High ROX)
- 蛋白實(shí)驗(yàn)
- Protein A/G免疫沉淀磁珠
- Anti-DYKDDDDK Tag免疫磁珠
- Anti-DYKDDDDK Tag親和凝膠
- Anti-Myc免疫磁珠
- Anti-HA免疫磁珠
- 磁力架
- Poly DYKDDDDK Tag多肽
- 細(xì)胞核與細(xì)胞漿蛋白抽提試劑盒
- 免疫磁珠
- 蛋白酶抑制劑Cocktail
- 蛋白酶抑制劑Cocktail(DMSO儲(chǔ)液)
- 磷酸酶抑制劑Cocktail
- 細(xì)胞實(shí)驗(yàn)
- Cell Counting Kit-8 (CCK-8)
- 動(dòng)物實(shí)驗(yàn)
- 鼠尾直接PCR試劑盒(快速基因型鑒定)
- 小鼠CD3+ T細(xì)胞分選試劑盒
- 小鼠CD4+ T細(xì)胞分選試劑盒
- 小鼠CD8+ T細(xì)胞分選試劑盒
- 新產(chǎn)品
- 聯(lián)系我們
Luteolin
別名: Luteoline, Luteolol, Digitoflavone 中文名稱：木犀草素，四羥黃酮
Luteolin (Luteoline, Luteolol, Digitoflavone)是一種非選擇性的PDE抑制劑，作用于PDE1-5，Ki分別為15.0 μM， 6.4 μM， 13.9 μM， 11.1 μM和9.5 μM。
Luteolin Chemical Structure
CAS: 491-70-3
產(chǎn)品質(zhì)控
批次：
純度：
99.18%
99.18
Luteolin相關(guān)產(chǎn)品
| 相關(guān)產(chǎn)品 | Camptothecin (CPT) SN-38 Triptolide (+)-Bicuculline Rutin BHQ Pinocembrin Lappaconite HBr Psoralen Harmine hydrochloride Lycorine hydrochloride Tetrahydropalmatine hydrochloride Luteoloside | 點(diǎn)擊展開(kāi) |
|---|---|---|
| 相關(guān)化合物庫(kù) | 代謝化合物庫(kù) 抗癌代謝化合物庫(kù) 谷氨酰胺代謝化合物庫(kù) 糖代謝化合物庫(kù) 脂代謝化合物庫(kù) | 點(diǎn)擊展開(kāi) |
細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例
| 細(xì)胞系 | 實(shí)驗(yàn)類型 | 給藥濃度 | 孵育時(shí)間 | 活性描述 | 文獻(xiàn)信息(PMID) |
|---|---|---|---|---|---|
| human THP1 cells | Function assay | 20 μM | 1 h | Downregulation of TPA-induced NOX2 mRNA expression in human THP1 cells at 20 uM incubated for 1 hr prior to TPA challenge measured after 24 hrs by RT-PCR analysis | |
| MDA-MB-231 cells | Function assay | 5 μM | 16 h | Inhibition of PMA-stimulated NF-kappaB signaling (unknown origin) expressed in MDA-MB-231 cells at 5 uM incubated for 16 hrs by luciferase reporter gene assay | |
| HL60 cells | Proliferation assay | 30 μM | 48 h | Antiproliferative activity against human HL60 cells at 30 uM after 48 hrs by MTS assay | |
| mouse RAW264.7 cells | Function assay | 2 h | Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells pre-incubated for 2 hrs before LPS stimulation for 24 hrs by Griess assay method, IC50=0.21 μM | ||
| human MV4-11 cells | Cytotoxicity assay | 72 h | Cytotoxicity against human MV4-11 cells harboring FLT3 mutation after 72 hrs by tetrazolium based Ez CyTox cell viability assay, GI50=1.76 μM | ||
| HEK293 cells | Function assay | 24 h | Agonist activity at mouse PPARgamma expressed in HEK293 cells co-expressing with Gal4 reporter vector after 24 hrs by dual-luciferase reporter assay, EC50=2.3 μM | ||
| human U2OS cells | Function assay | 5 h | Agonist activity at GPR35 receptor in human U2OS cells coexpressing Gal4-VP16-TEV assessed as beta arrestin translocation after 5 hrs by beta lactamase reporter gene assay, EC50=3.2 μM | ||
| rat H9c2 cells | Function assay | 24 h | Cytoprotective activity against doxorubicin-induced cytotoxicity in rat H9c2 cells assessed as cell viability after 24 hrs by MTT assay, EC50=5.53 μM | ||
| human HT-29 cells | Function assay | 10 mins | Agonist activity at GPR35 receptor in human HT-29 cells after 10 mins by dynamic mass redistribution assay, EC50=7.24 μM | ||
| human RS4:11 cells | Cytotoxicity assay | 72 h | Cytotoxicity against human RS4:11 cells harboring wild type FLT3 after 72 hrs by tetrazolium based Ez CyTox cell viability assay, GI50=7.25 μM | ||
| NCI-H460 cells | Function assay | 2-20 h | Inhibition of ABCG2 expressed in human NCI-H460 cells assessed as inhibition of PhA accumulation after 2 to 20 hrs relative to fumitremorgin C, IC50=8.9 μM | ||
| human H9 cells | Function assay | 3 days | Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay, EC50=10 μM | ||
| mouse B16-4A5 cells | Function assay | 72 h | Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs, IC50=14 μM | ||
| K562 cells | Growth inhibition assay | 5 days | Growth inhibition of K562 cells by XTT assay after 5 days, IC50=14.65 μM | ||
| human H9 cells | Cytotoxicity assay | 3 days | Cytotoxicity against human H9 cells after 3 days, IC50=16 μM | ||
| human HT-29 cells | Function assay | 10 mins | Desensitization of GPR35 receptor in human HT-29 cells assessed as inhibition of zaprinast-induced dynamic mass redistribution after 10 mins, IC50=18.6 μM | ||
| human U937 cells | Proliferation assay | 72 h | Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay, IC50=20 μM | ||
| mouse HT22 cells | Function assay | 3 h | Neuroprotective activity in mouse HT22 cells assessed as t-BOOH-induced toxicity at 40 uM preincubated for 3 hrs followed by t-BOOH induction measured after 9 hrs by MTT assay | ||
| HEK293 FS cells | Function assay | Inhibition of NOX4 expressed in HEK293 FS cells assessed as H2O2 production by H2O2/Tyr/LPO assay, IC50=0.85 μM | |||
| CHO cells | Function assay | Agonist activity at rat DAT expressed in CHO cells, EC50=1.45 μM | |||
| RBL-2H3 cells | Function assay | Inhibitory activity against IL-4 production in RBL-2H3 cells was determined, IC50=3.7 μM | |||
| human mast cells | Function assay | Inhibition of SYK in human mast cells assessed as reduction in mast cell degranulation, EC50=4.5 μM | |||
| human LNCAP cells | Function assay | Downregulation of prostate specific antigen secretion in human LNCAP cells, IC50=5 μM | |||
| MDCK cells | Cytotoxicity assay | Cytotoxicity against MDCK cells by MTT assay, CC50=12.44 μM | |||
| 點(diǎn)擊查看更多細(xì)胞系數(shù)據(jù) | |||||
生物活性
| 產(chǎn)品描述 | Luteolin (Luteoline, Luteolol, Digitoflavone)是一種非選擇性的PDE抑制劑，作用于PDE1-5，Ki分別為15.0 μM， 6.4 μM， 13.9 μM， 11.1 μM和9.5 μM。 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 靶點(diǎn) |
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| 體外研究(In Vitro) | ||||
| 體外研究活性 | Luteolin是在訶子中發(fā)現(xiàn)的黃酮類化合物，是非選擇性的磷酸二酯酶PDE抑制劑，對(duì)PDE1-5的Ki值分別為15.0 μM，6.4 μM，13.9 μM，11.1 μM以及9.5 μM。[1] Luteolin抑制LPS刺激的TNF-α的產(chǎn)生，IC50值低于1 μM。Luteolin抑制LPS誘導(dǎo)的Akt和IkappaBalpha的磷酸化。[2] |
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|---|---|---|---|---|
| 實(shí)驗(yàn)圖片 | 檢測(cè)方法 | 檢測(cè)指標(biāo) | 實(shí)驗(yàn)圖片 | PMID |
| Western blot | FADD / PARP / Cleaved PARP Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 p21 / Survivin / Cyclin D1 DNMT1 / DMNT3A / DNMT3B TET1 / TET2 / TET3 |
|
30992674 | |
| Immunofluorescence | 5-hmC |
|
30988303 | |
| Growth inhibition assay | Cell viability |
|
30992674 | |
| 體內(nèi)研究(In Vivo) | ||
| 體內(nèi)研究活性 | LD50:小鼠>2500毫克/千克(i.g.) [3] |
|
|---|---|---|
|
化學(xué)信息&溶解度
| 分子量 | 286.24 | 分子式 | C15H10O6 |
| CAS號(hào) | 491-70-3 | SDF | Download Luteolin SDF |
| 儲(chǔ)存條件(自收到貨起) | |||
|
體外溶解度 |
DMSO : 57 mg/mL ( (199.13 mM) ；DMSO吸濕會(huì)降低化合物溶解度，請(qǐng)使用新開(kāi)封DMSO) Ethanol : 20 mg/mL (69.87 mM) Water : Insoluble |
摩爾濃度計(jì)算器 |
|
體內(nèi)溶解配方 現(xiàn)配現(xiàn)用，請(qǐng)按從左到右的順序依次添加，澄清后再加入下一溶劑 |
動(dòng)物體內(nèi)配方計(jì)算器 | |||||
實(shí)驗(yàn)計(jì)算
摩爾濃度計(jì)算器
動(dòng)物體內(nèi)配方計(jì)算器（澄清溶液）
第一步：請(qǐng)輸入基本實(shí)驗(yàn)信息（考慮到實(shí)驗(yàn)過(guò)程中的損耗，建議多配一只動(dòng)物的藥量）
mg/kg
g
μL
只
第二步：請(qǐng)輸入動(dòng)物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請(qǐng)聯(lián)系Selleck為您提供正確的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
計(jì)算結(jié)果：
工作液濃度： mg/ml；
DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過(guò)該批次藥物DMSO溶解度，請(qǐng)先聯(lián)系Selleck）；
體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH2O，混勻澄清。
體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。
注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。
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在訂購(gòu)、運(yùn)輸、儲(chǔ)存和使用我們的產(chǎn)品的任何階段，您遇到的任何問(wèn)題，均可以通過(guò)撥打我們的熱線電話400-668-6834，或者技術(shù)支持郵箱[email protected]，直接聯(lián)系到我們。我們會(huì)在24小時(shí)內(nèi)盡快聯(lián)系您。
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* 必填項(xiàng)
常見(jiàn)問(wèn)題及建議解決方法
問(wèn)題 1:
Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?
回答：
Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.
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