- 抑制劑
- 化合物庫
- 抗體
- 生物試劑
- qPCR
- 2x SYBR Green qPCR Master Mix
- 2x SYBR Green qPCR Master Mix(Low ROX)
- 2x SYBR Green qPCR Master Mix(High ROX)
- 蛋白實(shí)驗(yàn)
- Protein A/G免疫沉淀磁珠
- Anti-DYKDDDDK Tag免疫磁珠
- Anti-DYKDDDDK Tag親和凝膠
- Anti-Myc免疫磁珠
- Anti-HA免疫磁珠
- 磁力架
- Poly DYKDDDDK Tag多肽
- 細(xì)胞核與細(xì)胞漿蛋白抽提試劑盒
- 免疫磁珠
- 蛋白酶抑制劑Cocktail
- 蛋白酶抑制劑Cocktail(DMSO儲液)
- 磷酸酶抑制劑Cocktail
- 細(xì)胞實(shí)驗(yàn)
- Cell Counting Kit-8 (CCK-8)
- 新產(chǎn)品
- 聯(lián)系我們
Home
Microbiology
Anti-infection chemical
-
Caspase activator
-
PARP modulator
-
P450 (e.g. CYP17) activator
-
IAP inhibitor
-
Bcl-2 inhibitor
-
p38 MAPK activator
-
JNK activator
-
ROS activator
-
Apoptosis related activator
-
Autophagy modulator
-
Topoisomerase inhibitor
Berberine chloride
僅限科研使用
Berberine chloride
別名: NSC 646666, Natural Yellow 18 chloride 中文名稱：鹽酸小檗堿，黃連素
Berberine chloride是異喹啉類生物堿的季銨鹽。Berberine chloride 可激活 caspase 3 和 caspase 8，分裂 poly ADP-ribose polymerase (PARP) 和釋放 cytochrome c。Berberine chloride 可降低 c-IAP1，Bcl-2 和 Bcl-XL 的表達(dá)。Berberine chloride 可通過 JNK 和 p38 MAPK 的持續(xù)磷酸化以及產(chǎn)生 ROS 來誘導(dǎo)凋亡。Berberine chloride 是一個 topoisomerase I 和 II 的雙效抑制劑。Berberine chloride 也是一個潛在的自噬調(diào)節(jié)劑。
Berberine chloride Chemical Structure
CAS: 633-65-8
產(chǎn)品質(zhì)控
批次：
純度：
99.75%
99.75
Berberine chloride相關(guān)產(chǎn)品
細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例
| 細(xì)胞系 | 實(shí)驗(yàn)類型 | 給藥濃度 | 孵育時間 | 活性描述 | 文獻(xiàn)信息(PMID) |
|---|---|---|---|---|---|
| MRC5 cells | Proliferation assay | 10 μM | 54 hrs | Inhibition of HCMV proliferation in MRC5 cells after 54 hrs post-infection at 10 uM by plaque assay | |
| MRC5 cells | Proliferation assay | 10 μM | 24 h | Inhibition of HCMV proliferation in MRC5 cells after 24 hrs post-infection at 10 uM by plaque assay | |
| HepG2 cells | Function assay | 10 ug/mL | 12 h | Induction of LDLR protein expression in human HepG2 cells at 10 ug/mL after 12 hrs by flow cytometry | |
| A10 cells | Function assay | 30 μM | 24 hrs | Downregulation of Scd2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis | |
| A10 cells | Function assay | 30 μM | 24 hrs | Down regulation of Prim2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis | |
| A10 cells | Function assay | 30 μM | 24 hrs | Downregulation of Impk mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis | |
| HepG2 cells | Function assay | 10 μM | 4 h | Increase in AMPKalpha phosphorylation in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control | |
| HepG2 cells | Function assay | 10 μM | 4 h | Increase in total AMPKalpha level in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control | |
| HepG2 cells | Function assay | 20 μM | 24 hrs | Induction of apoptosis in human HepG2 cells assessed as morphological changes at 20 uM after 24 hrs using Hoechst 33258 staining by fluorescence microscopic analysis | |
| HepG2-A16-CD81 cells | Function assay | 10 μM | NOVARTIS: Antimalarial liver stage activity measured as a greater than 50% reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells at 10uM compound concentration, determined by immuno-fluorescence. | ||
| HepG2-A16-CD81 cells | Function assay | 10 μM | NOVARTIS: Antimalarial liver stage activity measured as reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells by immuno-fluorescence, and median schizont size at 10uM compound concentration, IC50=0.548 μM | ||
| Bel7402 cells | Function assay | 12 h | Induction of LDLR protein in human Bel7402 cells after 12 hrs by RT-PCR assay relative to control | ||
| KB cells | Cytotoxicity assay | 72 h | Cytotoxicity against human KB cells after 72 hrs, IC50=7.32 μM | ||
| HL60 cells | Apoptosis assay | 48 hrs | Induction of apoptosis in human HL60 cells after 48 hrs using annexin V-propidium iodide staining by FACS analysis | ||
| CEM cells | Cytotoxicity assay | 48 hrs | Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay, CC50=2.09 μM | ||
| human CEM cells | Function assay | 7 days | Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA, EC50=0.13 μM | ||
| SKN cells | Growth inhibition assay | 72 h | Growth inhibition against human SKN cells after 72 hrs by MTT assay, GI50=15.88 μM | ||
| RKN cells | Growth inhibition assay | 48 hrs | Growth inhibition against human RKN cells after 48 hrs by MTT assay, GI50=49.6 μM | ||
| G402 cells | Growth inhibition assay | 48 hrs | Growth inhibition against human G402 cells after 48 hrs by MTT assay, GI50=11.87 μM | ||
| HT-29 cells | Cytotoxicity assay | 48 hrs | Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay, IC50=8.45 μM | ||
| HepG2 cells | Cytotoxicity assay | 24 hrs | Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50=11.22 μM | ||
| HepG2 cells | Cytotoxicity assay | 48 hrs | Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay, IC50=8.32 μM | ||
| MRC5 cells | Function assay | Antiviral activity against HCMV in MRC5 cells by plaque reduction assay, IC50=0.68 μM | |||
| A549 cells | Cytotoxicity assay | Cytotoxicity against human A549 cells by SRB assay, IC50=6.27 μM | |||
| SKOV3 cells | Cytotoxicity assay | Cytotoxicity against human SKOV3 cells by SRB assay, IC50=16.44 μM | |||
| SK-MEL-2 cells | Cytotoxicity assay | Cytotoxicity against human SK-MEL-2 cells by SRB assay, IC50=13.76 μM | |||
| HCT15 cells | Cytotoxicity assay | Cytotoxicity against human HCT15 cells by SRB assay, IC50=16.59 μM | |||
| 點(diǎn)擊查看更多細(xì)胞系數(shù)據(jù) | |||||
生物活性
| 產(chǎn)品描述 | Berberine chloride是異喹啉類生物堿的季銨鹽。Berberine chloride 可激活 caspase 3 和 caspase 8，分裂 poly ADP-ribose polymerase (PARP) 和釋放 cytochrome c。Berberine chloride 可降低 c-IAP1，Bcl-2 和 Bcl-XL 的表達(dá)。Berberine chloride 可通過 JNK 和 p38 MAPK 的持續(xù)磷酸化以及產(chǎn)生 ROS 來誘導(dǎo)凋亡。Berberine chloride 是一個 topoisomerase I 和 II 的雙效抑制劑。Berberine chloride 也是一個潛在的自噬調(diào)節(jié)劑。 | ||||||
|---|---|---|---|---|---|---|---|
| 靶點(diǎn) |
|
| NCT Number | Recruitment | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|
| NCT06273241 | Not yet recruiting | Pharmacokinetic Study in Healthy Volunteers |
University Medicine Greifswald |
March 4 2024 | Not Applicable |
| NCT05845931 | Recruiting | Pharmacokinetic Study in Healthy Volunteers |
University Medicine Greifswald |
May 5 2023 | Not Applicable |
| NCT05480670 | Completed | Polycystic Ovary Syndrome |
Ayub Teaching Hospital |
November 1 2022 | Not Applicable |
| NCT05463003 | Completed | Pharmacokinetic Study in Healthy Volunteers |
University Medicine Greifswald |
July 19 2022 | Not Applicable |
|
化學(xué)信息&溶解度
| 分子量 | 371.81 | 分子式 | C20H18NO4.Cl |
| CAS號 | 633-65-8 | SDF | Download Berberine chloride SDF |
| 儲存條件(自收到貨起) | |||
|
體外溶解度 |
DMSO : 25 mg/mL ( (67.23 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO) Water : Insoluble Ethanol : Insoluble |
摩爾濃度計算器 |
|
體內(nèi)溶解配方 現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑 |
動物體內(nèi)配方計算器 | |||||
實(shí)驗(yàn)計算
動物體內(nèi)配方計算器（澄清溶液）
第一步：請輸入基本實(shí)驗(yàn)信息（考慮到實(shí)驗(yàn)過程中的損耗，建議多配一只動物的藥量）
mg/kg
g
μL
只
第二步：請輸入動物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
計算結(jié)果：
工作液濃度： mg/ml；
DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；
體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH2O，混勻澄清。
體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。
注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。
技術(shù)支持
在訂購、運(yùn)輸、儲存和使用我們的產(chǎn)品的任何階段，您遇到的任何問題，均可以通過撥打我們的熱線電話400-668-6834，或者技術(shù)支持郵箱[email protected]，直接聯(lián)系到我們。我們會在24小時內(nèi)盡快聯(lián)系您。
如果有其他問題，請給我們留言。
* 必填項(xiàng)
