Puromycin Dihydrochloride

別名: CL13900 2HCl 中文名稱：嘌呤霉素鹽酸鹽

目錄號：S7417 Purity: 99.23%

Puromycin 2HCl是一種源自Streptomyces alboniger的氨基核苷類抗生素。它是蛋白質(zhì)合成的抑制劑，已用于研究細胞分化過程中控制基因順序和協(xié)調(diào)表達的轉(zhuǎn)錄調(diào)控機制。

Puromycin Dihydrochloride Chemical Structure

CAS: 58-58-2

規(guī)格	價格	庫存
10mM (1mL in DMSO)	573.3	現(xiàn)貨
25mg	794.9	現(xiàn)貨
100mg	2350.85	現(xiàn)貨
500mg	6306.3	現(xiàn)貨
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產(chǎn)品質(zhì)控

批次：純度： 99.23%

99.23

常與Puromycin Dihydrochloride一起在實驗中被使用的化合物

Blasticidin S Hydrochloride

Puromycin和Blasticidin S抑制轉(zhuǎn)移性去勢抵抗性前列腺癌(mCRPC)細胞PC3/DU145的增殖和細胞活力。

Melatonin

Melatonin通過激活HL-60細胞中的caspase-3和AMPK來增強嘌呤霉素誘導(dǎo)的細胞凋亡。

Bortezomib

Puromycin和Bortezomib的組合顯著增加了多發(fā)性骨髓瘤小鼠模型中Bortezomib的抗腫瘤活性。

Glycocholic acid

Puromycin和Glycocholate組合可增強亮氨酸腦啡肽(Leu-Enk)的吸收。

Doxorubicin

在p53野生型HCT116細胞中，Purromycin和Doxorubicin通過抑制c-Myc和Bcl-2以及激活p53來促進細胞凋亡

Puromycin Dihydrochloride相關(guān)產(chǎn)品

相關(guān)產(chǎn)品	Staurosporine (STS) Oligomycin A (MCH 32) Geldanamycin Honokiol Streptozotocin (STZ) Sodium Monensin (NSC 343257) Cephalomannine 10-Deacetylbaccatin-III Pirarubicin Hygromycin B Roxithromycin	點擊展開
相關(guān)化合物庫	抗感染化合物庫抗生素化合物庫抗病毒化合物庫抗寄生蟲藥物庫腸道微生物代謝物庫	點擊展開

細胞實驗數(shù)據(jù)示例

細胞系	實驗類型	孵育時間	活性描述	文獻信息(PMID)
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM.	18512984
K562	Growth inhibition assay	72 h	Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM.	18313307
K562	Growth inhibition assay	72 h	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM.	18313307
HEK239	Cytotoxicity assay	72 h	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM.	20558060
KB3-1	Growth inhibition assay	72 hrs	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM.	18313307
HepG2/Dox	Growth inhibition assay	72 hrs	Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM.	18512984
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM.	26651537
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM.	27060763
HEK293	Growth inhibition assay	72 hrs	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM.	28001067
SU-DHL10	Cytotoxicity assay	72 hrs	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM.	28523103
MIAPaCa2	Cytotoxicity assay	72 hrs	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM.	28523103
MDA-MB-231	Cytotoxicity assay	72 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM.	28523103
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM.	28774427
HL60	Antiparasitic assay	72 hrs	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM.	28803047
MOLT4	Antiparasitic assay	72 hrs	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM.	28803047
Vero	Cytotoxicity assay	72 hrs	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM.	28803047
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM.	29236492
HEK293T	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM.	29469575
HepG2	Cytotoxicity assay	72 hrs	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM.	29469575
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM.	29533611
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM.	29969262
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
Huh7	Cytotoxicity assay		NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM.	18579783
J774A1	Cytotoxicity assay		Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM.	22934636
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells, IC50 = 0.6 μM.	27089214
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells, IC50 = 0.7 μM.	27089214
HEK293	Cytotoxicity assay		Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM.	27212070
HEK293	Growth inhibition assay		Growth inhibition of HEK293 cells, IC50 = 0.399 μM.	28400231
HCT8	Cytotoxicity assay		Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM.	29469575
點擊查看更多細胞系數(shù)據(jù)

生物活性

產(chǎn)品描述	Puromycin 2HCl是一種源自Streptomyces alboniger的氨基核苷類抗生素。它是蛋白質(zhì)合成的抑制劑，已用于研究細胞分化過程中控制基因順序和協(xié)調(diào)表達的轉(zhuǎn)錄調(diào)控機制。

體外研究(In Vitro)
體外研究活性	抗生素puromycin由actinoniycete和Streptornyces alboniger產(chǎn)生，在許多體系中被用作研究蛋白質(zhì)合成的工具。^[1] Puromycin能夠用于從非培養(yǎng)細胞中選擇重組細胞。^[2]
實驗圖片	檢測方法	檢測指標	實驗圖片	PMID
	Western blot	Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc		31022952
	Growth inhibition assay	Cell viability		31022952

參考文獻
[1]https://pubmed.ncbi.nlm.nih.gov/14211122/ [2]https://pubmed.ncbi.nlm.nih.gov/15385422/ [3]https://pubmed.ncbi.nlm.nih.gov/28971619/ [4]https://pubmed.ncbi.nlm.nih.gov/3953770/ [5]https://pubmed.ncbi.nlm.nih.gov/32435426/ [6]https://pubmed.ncbi.nlm.nih.gov/11397778/ + 展開

參考文獻

[1]https://pubmed.ncbi.nlm.nih.gov/14211122/
[2]https://pubmed.ncbi.nlm.nih.gov/15385422/
[3]https://pubmed.ncbi.nlm.nih.gov/28971619/

[4]https://pubmed.ncbi.nlm.nih.gov/3953770/
[5]https://pubmed.ncbi.nlm.nih.gov/32435426/
[6]https://pubmed.ncbi.nlm.nih.gov/11397778/

+ 展開

化學信息&溶解度

分子量	544.43	分子式	C₂₂H₂₉N₇O₅.2HCl
CAS號	58-58-2	SDF	Download Puromycin Dihydrochloride SDF
Smiles	CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復(fù)凍融！	1年-80°C溶于溶劑
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復(fù)凍融！	1個月-20°C溶于溶劑

體外溶解度
批次：

DMSO : 100 mg/mL ( (183.67 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : 100 mg/mL (183.67 mM)

Ethanol : Insoluble

DMSO : 100 mg/mL ( (183.67 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : 100 mg/mL (183.67 mM)

Ethanol : Insoluble

DMSO : 100 mg/mL ( (183.67 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : 100 mg/mL (183.67 mM)

Ethanol : Insoluble

摩爾濃度計算器

體內(nèi)溶解配方批次：現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑				動物體內(nèi)配方計算器
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。

實驗計算

摩爾濃度計算器

質(zhì)量	濃度	體積	分子量
=	×	×

稀釋計算器分子量計算器

動物體內(nèi)配方計算器（澄清溶液）

第一步：請輸入基本實驗信息（考慮到實驗過程中的損耗，建議多配一只動物的藥量）

給藥劑量 mg/kg 動物平均體重 g 每只動物給藥體積 μL 動物數(shù)量只

第二步：請輸入動物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

計算結(jié)果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；

體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH₂O，混勻澄清。

體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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