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DNA Damage/DNA Repair
Antineoplastic and Immunosuppressive Antibiotics inhibitor
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DNA/RNA Synthesis inhibitor
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DNA alkylator inhibitor
Carboplatin
僅限科研使用
Carboplatin
別名: NSC 241240, JM-8, CBDCA 中文名稱：卡鉑
鉑類藥物不建議使用DMSO溶解，易失活！
Carboplatin是 CDDP 的衍生物，是一種 DNA 合成抑制劑，在 A2780、SKOV-3、IGROV-1 和 HX62 細胞中可與 DNA 結(jié)合，抑制復(fù)制和轉(zhuǎn)錄，誘導細胞死亡并干擾細胞的修復(fù)機制發(fā)揮作用。它誘導 DNA 鏈間交聯(lián)和 DNA-蛋白質(zhì)交聯(lián)的形成。在溶液中不穩(wěn)定，請現(xiàn)配現(xiàn)用！鉑類藥物不建議用DMSO溶解，易失活！
Carboplatin Chemical Structure
CAS: 41575-94-4
產(chǎn)品質(zhì)控
批次：
純度：
99.98%
99.98
Carboplatin相關(guān)產(chǎn)品
細胞實驗數(shù)據(jù)示例
| 細胞系 | 實驗類型 | 給藥濃度 | 孵育時間 | 活性描述 | 文獻信息(PMID) |
|---|---|---|---|---|---|
| H460? | Growth Inhibition Assay | 7.9 μM | 24 h | shows a radiosensitizing effect to X-rays | 25599995 |
| A549/CDDP | Growth Inhibition Assay | 0.2-4000 μM | 72 h | IC50=240?±?45.7 μM | 25625243 |
| A549 | Growth Inhibition Assay | 0.2-4000 μM | 72 h | IC50=126 ± 5.0 μM | 25625243 |
| A-549? | Fuction Assay | 0.5 μM | 48 h | inhibits of cell migration? | 25755690 |
| A-549? | Fuction Assay | 0.5 μM | 48 h | shows aberrant microtubule disruption | 25755690 |
| A-549? | Apoptosis Assay | 0.5 μM | 48 h | shows early apoptosis features including blebbing and chromatin condensation | 25755690 |
| A-549? | Growth Inhibition Assay | 0.5 μM | 24 h | inhibits cell growth significantly | 25755690 |
| MCF7 | Growth Inhibition Assay | 20 μM | 72 h | induces cell death of HP1β-depleted MCF7 cells | 25769025 |
| T47D | Growth Inhibition Assay | 0-220 μg/ml | 72 h | IC50?= 48.9 μM | 25837691 |
| MDA-MB-231 | Growth Inhibition Assay | 0-220 μg/ml | 72 h | IC50?= 86 μM | 25837691 |
| H1975 | Growth Inhibition Assay | 25 μM | 24 h | inhibits cell growth | 25917317 |
| A549 | Growth Inhibition Assay | 25 μM | 24 h | inhibits cell growth | 25917317 |
| H1975 | Fuction Assay | 0/25/50 μM | 24 h | upregulates miR-218 expression | 25917317 |
| A549 | Fuction Assay | 0/25/50 μM | 24 h | upregulates miR-218 expression | 25917317 |
| H1975 | Fuction Assay | 0/25/50 μM | 24 h | downregulates miR-205 expression? | 25917317 |
| A549 | Fuction Assay | 0/25/50 μM | 24 h | downregulates miR-205 expression? | 25917317 |
| SKOV3 | Apoptosis Assay | 2 μg/ml | 48 h | induces apoptosis | 26125273 |
| SKOV3 | Growth Inhibition Assay | 0-4 μg/ml | 24/48/72 h | inhibits cell viability dose and time dependently | 26125273 |
| SKOV3 | Growth Inhibition Assay | 60 mg/ml | 1-5 d | inhibits cell viability time dependently | 26137043 |
| Tca8113/PYM? | Growth Inhibition Assay | 2-25?μM | 72 h | inhibits cell viability dose dependently | 26137105 |
| Tca8113/CBP? | Growth Inhibition Assay | 2-25?μM | 72 h | inhibits cell viability dose dependently | 26137105 |
| Tca8113/ut? | Growth Inhibition Assay | 2-25?μM | 72 h | inhibits cell viability dose dependently | 26137105 |
| PC9 | Apoptosis Assay | 100 μM | 72 h | induces apoptosis | 24618809 |
| A549? | Apoptosis Assay | 100 μM | 72 h | induces apoptosis | 24618809 |
| IMR-32 | Fuction Assay | 10 μM? | induces apoptosis significantly combined with GSK2830371 | 25658463 | |
| CHP-134? | Fuction Assay | 10 μM? | induces apoptosis significantly combined with GSK2830371 | 25658463 | |
| IMR-32 | Growth Inhibition Assay | 1 μM | inhibits significant cell growth combined with GSK2830371 | 25658463 | |
| CHP-134? | Growth Inhibition Assay | 1 μM | inhibits significant cell growth combined with GSK2830371 | 25658463 | |
| OSA | Clonogenic Assay | 2.5/5 μM | results in lower colony formation as compared to either BMI1 siRNA treatment | 26110620 | |
| HeLa | Growth Inhibition Assay | 5-80 μM | inhibits cell growth dose dependently | 25109360 | |
| MCF-7 | Growth Inhibition Assay | 5-80 μM | inhibits cell growth dose dependently | 25109360 | |
| MDA-MB-231 | Growth Inhibition Assay | 5-80 μM | inhibits cell growth dose dependently | 25109360 | |
| OVCAR-3 | Growth Inhibition Assay | 72 h | IC50=32.6 μM | 25520132 | |
| SW626 | Growth Inhibition Assay | 72 h | IC50=97.0 μM | 25520132 | |
| A2780 | Growth Inhibition Assay | 72 h | IC50=145.7 μM | 25520132 | |
| 3AO | Growth Inhibition Assay | 72 h | IC50=63.4 μM | 25520132 | |
| OVCAR-8 | Growth Inhibition Assay | 72 h | IC50=96.0 μM | 25520132 | |
| ES-2 | Growth Inhibition Assay | 72 h | IC50=14.6 μM | 25520132 | |
| SK-OV-3 | Growth Inhibition Assay | 72 h | IC50=104.8 μM | 25520132 | |
| RGE | Growth Inhibition Assay | 72?h? | IC80=63 ± 7.4 μM | 25565603 | |
| NRK-52E | Growth Inhibition Assay | 72?h? | IC80=179 ± 9.5 μM | 25565603 | |
| RGE | Growth Inhibition Assay | 72?h? | IC50=22 ± 7.4 μM | 25565603 | |
| NRK-52E | Growth Inhibition Assay | 72?h? | IC50=100 ± 12.9 μM | 25565603 | |
| H460? | Growth Inhibition Assay | 24 h | IC50=7.9 μM | 25599995 | |
| CaOV3 | Growth Inhibition Assay | 72 h | IC50=86.1 μM | 25068849 | |
| OVCAR-3 | Growth Inhibition Assay | 72 h | IC50=179.5 μM | 25068849 | |
| OVCAR-5 | Growth Inhibition Assay | 72 h | IC50=75.7 μM | 25068849 | |
| SKOV-3 | Growth Inhibition Assay | 72 h | IC50=504 μM | 25068849 | |
| OVCAR-4 | Growth Inhibition Assay | 72 h | IC50=290.6 μM | 25068849 | |
| OVCAR-8 | Growth Inhibition Assay | 72 h | IC50=690.2 μM | 25068849 | |
| MA148 | Growth Inhibition Assay | 24 h | IC50=83.6?±?1.4 μg/ml | 24831091 | |
| A549 | Growth Inhibition Assay | 24 h | IC50=776.0?±?1.9 μg/ml | 24831091 | |
| MDA-MB-231 | Growth Inhibition Assay | 24 h | IC50=748.0?±?1.1 μg/ml | 24831091 | |
| NCI-ADR/RES? | Growth Inhibition Assay | 24 h | IC50=1753.0?±?4.4 μg/ml | 24831091 | |
| MA148 | Growth Inhibition Assay | 48 h | IC50=28.1?±?1.0 μg/ml | 24831091 | |
| A549 | Growth Inhibition Assay | 48 h | IC50=118.0?±?1.2 μg/ml | 24831091 | |
| MDA-MB-231 | Growth Inhibition Assay | 48 h | IC50=183.0?±?1.2 μg/ml | 24831091 | |
| NCI-ADR/RES? | Growth Inhibition Assay | 48 h | IC50=193.0?±?1.9 μg/ml | 24831091 | |
| MA148 | Growth Inhibition Assay | 72 h | IC50=13.7?±?1.2 μg/ml | 24831091 | |
| A549 | Growth Inhibition Assay | 72 h | IC50=64.3?±?1.1 μg/ml | 24831091 | |
| MDA-MB-231 | Growth Inhibition Assay | 72 h | IC50=99.5?±?1.1 μg/ml | 24831091 | |
| NCI-ADR/RES? | Growth Inhibition Assay | 72 h | IC50=37.4?±?1.7 μg/ml | 24831091 | |
| A549 | Growth Inhibition Assay | 72 h | IC50=152.409 μM | 24721323 | |
| NCI-H157 | Growth Inhibition Assay | 72 h | IC50=89.977 μM | 24721323 | |
| PC9 | Growth Inhibition Assay | 72 h | GC50=71.6±9.5 μM | 24618809 | |
| A549? | Growth Inhibition Assay | 72 h | GC50=136±31.6 μM? | 24618809 | |
| A549 | Growth Inhibition Assay | 24 h | IC10=1.476 nM | 24396437 | |
| H1299 | Growth Inhibition Assay | 24 h | IC10=0.443 nM | 24396437 | |
| HCC15 | Growth Inhibition Assay | 24 h | IC10=0.346 nM | 24396437 | |
| H157 | Growth Inhibition Assay | 24 h | IC10=0.81 nM | 24396437 | |
| 4T1 | Growth Inhibition Assay | IC50=84.62 ± 30.05 μM μM | 25277461 | ||
| A549 | Growth Inhibition Assay | IC50=151.56 ± 53.06 μM | 25277461 | ||
| FN-RMG-1-hFUT | Growth Inhibition Assay | IC50=118.8 ± 13.8 mg/L | 25726913 | ||
| RMG-1-hFUT | Growth Inhibition Assay | IC50=58.1 ± 2.4 mg/L | 25726913 | ||
| FN-RMG-1 | Growth Inhibition Assay | IC50=40.5 ± 4.2 mg/L μM | 25726913 | ||
| RMG-1 | Growth Inhibition Assay | IC50=28.8 ± 3.6 mg/L | 25726913 | ||
| MonoTCC | Growth Inhibition Assay | IC50=5.6?μM | 25773167 | ||
| MegTCC | Growth Inhibition Assay | IC50=4.1?μM | 25773167 | ||
| MCTCC | Growth Inhibition Assay | IC50=2.8?μM | 25773167 | ||
| LCTCC | Growth Inhibition Assay | IC50=3.8?μM | 25773167 | ||
| BALB/3T3 | Growth Inhibition Assay | IC50=14.30 ± 6.68 μM | 25277461 | ||
| B16 | Growth Inhibition Assay | IC50=64.30 ± 6.81 μM | 25277461 | ||
| DU 145 | Growth Inhibition Assay | IC50=35.04 ± 17.54 μM | 25277461 | ||
| FaDu | Growth Inhibition Assay | IC50=45.39 ± 11.50 μM | 25277461 | ||
| HCV-29T | Growth Inhibition Assay | IC50=48.57 ± 22.49 μM | 25277461 | ||
| HL-60 | Growth Inhibition Assay | IC50=11.10 ± 4.47 μM | 25277461 | ||
| HT-29 | Growth Inhibition Assay | IC50=89.16 ± 18.86 μM | 25277461 | ||
| H146 | Growth Inhibition Assay | IC50=49.6±13.1 μM | 25124282 | ||
| H187 | Growth Inhibition Assay | IC50=12.7±2.7 μM | 25124282 | ||
| H128 | Growth Inhibition Assay | IC50=106.4±9.6 μM | 25124282 | ||
| H69 | Growth Inhibition Assay | IC50=76.9±27.8 μM | 25124282 | ||
| H209 | Growth Inhibition Assay | IC50=258.1±99.8 μM | 25124282 | ||
| DMS153 | Growth Inhibition Assay | IC50=57.8±9.2 μM | 25124282 | ||
| H526 | Growth Inhibition Assay | IC50=7.2±1.7 μM | 25124282 | ||
| DMS114 | Growth Inhibition Assay | IC50=85.8±12.8 μM | 25124282 | ||
| DMS53 | Growth Inhibition Assay | IC50=146.2±36.4 μM | 25124282 | ||
| A2780 | Growth Inhibition Assay | IC50=14.0?±?1.4 μM | 24209693 | ||
| A2780cisR | Growth Inhibition Assay | IC50=48.9?±?3.9 μM | 24209693 | ||
| A2780ZD0473R | Growth Inhibition Assay | IC50=64.6?±?3.2 μM | 24209693 | ||
| SKOV-3 | Growth Inhibition Assay | IC50=43.4?±?3.9 μM | 24209693 | ||
| 點擊查看更多細胞系數(shù)據(jù) | |||||
生物活性
| 產(chǎn)品描述 | Carboplatin是 CDDP 的衍生物，是一種 DNA 合成抑制劑，在 A2780、SKOV-3、IGROV-1 和 HX62 細胞中可與 DNA 結(jié)合，抑制復(fù)制和轉(zhuǎn)錄，誘導細胞死亡并干擾細胞的修復(fù)機制發(fā)揮作用。它誘導 DNA 鏈間交聯(lián)和 DNA-蛋白質(zhì)交聯(lián)的形成。在溶液中不穩(wěn)定，請現(xiàn)配現(xiàn)用！鉑類藥物不建議用DMSO溶解，易失活！ | |
|---|---|---|
| 特性 | 不建議使用DMSO溶解。[6] | |
| 靶點 |
|
| 體外研究(In Vitro) | ||||||
| 體外研究活性 | Carboplatin作用于一組人類卵巢癌細胞系，包括A2780, SKOV3,和 IGROV-1細胞，抑制細胞增殖,IC50分別為6.1 μM, 12.4 μM 和2.2 μM。[1] |
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|---|---|---|---|---|---|---|
| 細胞實驗 | 細胞系 | A2780, SKOV3, IGROV-1 和 HX62 | ||||
| 濃度 | 0 到 200 μM | |||||
| 孵育時間 | 72 小時 | |||||
| 方法 | MTT實驗: 指數(shù)生長的A2780, SKOV3, IGROV-1和 HX62卵巢癌細胞接種在96孔板中。加入一系列濃度的Carboplatin，再溫育72小時。每組實驗按一式三份進行。 Sulforhodamine B (SRB)實驗:指數(shù)生長的 A2780細胞接種在96孔板中。使用濃度不斷增加的17-AAG 和Carboplatin處理細胞96小時。使用濃度不斷增加的17-AAG或Carboplatin處理細胞 24小時，研究17-AAG 或 Carboplatin處理序列的效果。選中的第一種藥劑處理細胞24小時，確保A2780細胞使用第一種藥劑至少處理雙倍時間(18-24小時)。使用無菌磷酸鹽緩沖液沖洗細胞，然后補充培養(yǎng)基。然后，加入第二種藥劑 (第一次24小時未處理細胞的)或培養(yǎng)基，再處理96小時。所有實驗按一式三份進行。使用中位數(shù)效果分析方法按行之有效的原則分析聯(lián)合研究的效果。使用內(nèi)部電子表格計算聯(lián)用的效果。 |
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| 實驗圖片 | 檢測方法 | 檢測指標 | 實驗圖片 | PMID | ||
| Western blot |
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