SAG Hydrochloride

目錄號：S7779 Purity: 99.98%

SAG (Smoothened Agonist) HCl是一種細(xì)胞滲透性Smoothened (Smo)激動劑，其在Shh-LIGHT2細(xì)胞中的EC50約為3 nM。

SAG Hydrochloride Chemical Structure

CAS: 2095432-58-7

規(guī)格	價格	庫存
10mM (1mL in DMSO)	2432.43	現(xiàn)貨
2mg	954.43	現(xiàn)貨
5mg	2024.65	現(xiàn)貨
25mg	6110.42	現(xiàn)貨
100mg	7944.3	現(xiàn)貨
1g	57248.1	現(xiàn)貨
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產(chǎn)品質(zhì)控

批次：純度： 99.98%

99.98

SAG Hydrochloride相關(guān)產(chǎn)品

相關(guān)靶點(diǎn)	Hedgehog Smoothened GLI	點(diǎn)擊展開
相關(guān)產(chǎn)品	Cyclopamine (11-Deoxojervine) GANT61 Purmorphamine SANT-1 SAG BMS-833923 Taladegib (LY2940680) HPI-4 (Ciliobrevin A) PF-5274857 Jervine Ciliobrevin D MK-4101 JK184	點(diǎn)擊展開
相關(guān)化合物庫	激酶抑制劑庫 FDA藥物庫干細(xì)胞小分子化合物庫 GPCR小分子化合物庫干細(xì)胞分化化合物庫	點(diǎn)擊展開

細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例

細(xì)胞系	實(shí)驗(yàn)類型	孵育時間	活性描述	文獻(xiàn)信息(PMID)
HUVEC	Cytotoxicity assay	24 h	Cytotoxicity against HUVEC after 24 hrs by WST-1 assay, IC50=22 μM	22985958
mouse Shh-Light II cell	Function assay	48 hrs	Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay, EC50=0.0088 μM	22985958
Shh-Light II	Function assay	48 hrs	Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay, EC50 = 0.0618 μM.	22985958
C3H10T1/2	Function assay	6 days	Agonist activity at Smo in mouse C3H10T1/2 cells assessed as induction of cell differentiation into osteoblast incubated for 6 days by alkaline phosphatase assay, EC50 = 0.13 μM.	27429255
NIH/3T3	Function assay		Agonist activity at Smo in mouse NIH/3T3 cells transfected with Gli-dependent firefly luciferase reporter assessed as stimulation of Gli transcription factor, EC50 = 0.03 μM.	27429255
A673	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
Saos-2	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
BT-37	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
RD	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells	29435139
SK-N-SH	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells	29435139
BT-12	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
MG 63 (6-TG R)	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells	29435139
NB1643	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
OHS-50	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
SJ-GBM2	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
SK-N-MC	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
NB-EBc1	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
Rh18	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells	29435139
點(diǎn)擊查看更多細(xì)胞系數(shù)據(jù)

生物活性

產(chǎn)品描述

SAG (Smoothened Agonist) HCl是一種細(xì)胞滲透性Smoothened (Smo)激動劑，其在Shh-LIGHT2細(xì)胞中的EC50約為3 nM。

靶點(diǎn)

Smoothened ^[1]
(mouse cultured cell assay)

3 nM(EC50)

體外研究(In Vitro)
體外研究活性	SAG通過直接結(jié)合到Smo螺旋束調(diào)控Smo活性。^[1] SAG以GRK2依賴的方式誘導(dǎo)通過Gli的Smo-依賴性信號。^[2] SAG(1 nM)也會誘導(dǎo)神經(jīng)元和神經(jīng)膠質(zhì)前體細(xì)胞的增殖，而不影響新生細(xì)胞的分化模式。^[3]
實(shí)驗(yàn)圖片	檢測方法	檢測指標(biāo)	實(shí)驗(yàn)圖片	PMID
	Western blot	LC3I / LC3II		23504944
	Immunofluorescence	Smo acetylated alpha tubulin		25636740

體內(nèi)研究(In Vivo)
體內(nèi)研究活性	在成年大鼠海馬中，SAG (2.5 nM)腦室內(nèi)給藥顯著增加新生細(xì)胞的數(shù)量，并延長海馬細(xì)胞的存活時間。^[3] 在小鼠中，SAG (20 μg/g, i.p.)有效防止GC-誘導(dǎo)的新生小腦發(fā)育異常。^[4]
動物實(shí)驗(yàn)	Animal Models	大鼠
	Dosages	2.5 nM
	Administration	側(cè)腦室給藥

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試驗(yàn)信息 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT05937178	Recruiting	Hepatitis B Chronic	Huashan Hospital\|The First Affiliated Hospital of Anhui Medical University\|Anhui Provincial Hospital\|Beijing YouAn Hospital\|Peking University People''s Hospital\|Peking University First Hospital\|Xiamen Hospital of Traditional Chinese Medicine\|First Affiliated Hospital of Fujian Medical University\|LanZhou University\|Third Affiliated Hospital Sun Yat-Sen University\|First Affiliated Hospital of Guangxi Medical University\|The Affiliated Hospital Of Guizhou Medical University\|Hainan General Hospital\|Hebei Medical University Third Hospital\|The First Affiliated Hospital of Henan University of Traditional Chinese Medicine\|Henan Provincial People''s Hospital\|The Second Affiliated Hospital of Harbin Medical University\|Tongji Hospital\|Wuhan Union Hospital China\|Renmin Hospital of Wuhan University\|Central South University\|Xiangya Hospital of Central South University\|The First Hospital of Jilin University\|the Second Hospital of Nangjing\|The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School\|The First Affiliated Hospital with Nanjing Medical University\|The Affiliated Hospital of Xuzhou Medical University\|The First Affiliated Hospital of Nanchang University\|Shengjing Hospital\|Qingdao Sixth People''s Hospital\|Shandong Provincial Hospital\|The Second Hospital of Shandong University\|The First Affiliated Hospital of Shanxi Medical University\|Tang-Du Hospital\|First Affiliated Hospital Xi''an Jiaotong University\|Ruijin Hospital\|West China Hospital\|Tianjin Second People''s Hospital\|First Affiliated Hospital of Xinjiang Medical University\|The First People''s Hospital of Yunnan\|Shulan (Hangzhou) Hospital\|Zhejiang University\|Southwest Hospital China\|The Second Affiliated Hospital of Chongqing Medical University\|Sichuan Provincial People''s Hospital	January 31 2023	--
NCT05701592	Recruiting	Posterior Cruciate Ligament Tear	Konrad Malinowski MD\|Artromedical Konrad Malinowski Clinic	December 20 2022	--
NCT03645044	Active not recruiting	Hiv\|HBV Coinfection	University of Melbourne\|National Health and Medical Research Council Australia\|The University of Western Australia\|University of Adelaide\|The HIV Netherlands Australia Thailand Research Collaboration\|University of Malaya\|YR Gaitonde Centre for AIDS Research and Education\|Melbourne Health	May 24 2018	--

參考文獻(xiàn)
[1]https://pubmed.ncbi.nlm.nih.gov/12391318/ [2]https://pubmed.ncbi.nlm.nih.gov/16908539/ [3]https://pubmed.ncbi.nlm.nih.gov/20600593/ [4]https://pubmed.ncbi.nlm.nih.gov/22013124/ + 展開

參考文獻(xiàn)

[1]https://pubmed.ncbi.nlm.nih.gov/12391318/
[2]https://pubmed.ncbi.nlm.nih.gov/16908539/
[3]https://pubmed.ncbi.nlm.nih.gov/20600593/

[4]https://pubmed.ncbi.nlm.nih.gov/22013124/

+ 展開

化學(xué)信息&溶解度

分子量	526.52	分子式	C₂₈H₂₉Cl₂N₃OS
CAS號	2095432-58-7	SDF	Download SAG Hydrochloride SDF
Smiles	CNC1CCC(CC1)N(CC2=CC(=CC=C2)C3=CC=NC=C3)C(=O)C4=C(C5=CC=CC=C5S4)Cl.Cl
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復(fù)凍融！	1年-80°C溶于溶劑
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復(fù)凍融！	1個月-20°C溶于溶劑

體外溶解度
批次：

DMSO : 82 mg/mL ( (155.73 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : 50 mg/mL (94.96 mM)

Ethanol : 27 mg/mL (51.28 mM)

Water : 100 mg/mL (189.92 mM)

DMSO : 28 mg/mL ( (53.17 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Ethanol : Insoluble

Water : 100 mg/mL (189.92 mM)

DMSO : 71 mg/mL ( (134.84 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Ethanol : 40 mg/mL (75.97 mM)

DMSO : 100 mg/mL ( (189.92 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : 100 mg/mL (189.92 mM)

Ethanol : 100 mg/mL (189.92 mM)

DMSO : 100 mg/mL ( (189.92 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : 100 mg/mL (189.92 mM)

Ethanol : Insoluble

摩爾濃度計算器

體內(nèi)溶解配方批次：現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑				動物體內(nèi)配方計算器
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產(chǎn)品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
澄清溶液	water 該配方已經(jīng)過Selleck實(shí)驗(yàn)室實(shí)測。如果上述溶解方案不能滿足您的需求，可聯(lián)系Selleck銷售提供定制化測試。	100.000mg/ml (189.93mM)	以 1 mL 工作液為例，取 100mg該產(chǎn)品加到1ml ddH2O中，混合均勻使其澄清，請現(xiàn)配現(xiàn)用！

實(shí)驗(yàn)計算

摩爾濃度計算器

質(zhì)量	濃度	體積	分子量
=	×	×

稀釋計算器分子量計算器

動物體內(nèi)配方計算器（澄清溶液）

第一步：請輸入基本實(shí)驗(yàn)信息（考慮到實(shí)驗(yàn)過程中的損耗，建議多配一只動物的藥量）

給藥劑量 mg/kg 動物平均體重 g 每只動物給藥體積 μL 動物數(shù)量只

第二步：請輸入動物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

計算結(jié)果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；

體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH₂O，混勻澄清。

體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

SAG Hydrochloride

產(chǎn)品質(zhì)控

SAG Hydrochloride相關(guān)產(chǎn)品

相關(guān)信號通路圖

細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例

生物活性

化學(xué)信息&溶解度

實(shí)驗(yàn)計算

技術(shù)支持